Table of Contents
What is QuEChERS method?
From Wikipedia, the free encyclopedia. QuEChERS is a solid phase extraction method for detection of pesticide residues in food. The name is a portmanteau word formed from “quick, easy, cheap, effective, rugged, and safe”.
Who invented QuEChERS?
3.3. Since the invention of the QuEChERS method by Anastassiades, the chemist of the USDA, in 2003, the QuEChERS method has been widely used as a kind of pre-treatment technique integrating the extraction and purification of pesticide residues in various substrates.
How do you use QuEChERS?
Below are detailed procedures for each step of the QuEChERS Method.
- Step 1: Homogenization and Sampling.
- Step 2: Addition of Extraction Solvent.
- Step 3: Liquid Extraction.
- Step 4: Buffering and Drying.
- Step 5: Extraction.
- Step 6: Separation.
- Step 7: Decanting and Dispersive SPE (dSPE) Clean-Up.
- Step 8: Separation.
How do you pronounce QuEChERS?
The sample preparation approach of QuEChERS (pronounce “ketchers”) was originally designed to improve the efficiency of pesticide extraction from fruits and vegetables.
Which is the best method for QuEChERS and SPE?
QuEChERS Workflow overview and original methods 2. Method development for alternative matrices 3. SPE for polar compounds 4. SPE for non-polar compounds 5. SPE for ionic compounds 6. Questions 2 Filtration and Other Sample Preparation Techniques 3
What are the advantages and disadvantages of QuEChERS?
• Extraction/partitioning • Dispersive SPE • Simplicity of the sample preparation process • Analysis LC/MS/MS, GC/MS, and GC/MS/MS • QuEChERS modifications • Low water content products • Very polar compounds – QuEChERS: Thinking out of the sample preparation box 11/23/2010 Sample Preparation • LLE: liquid liquid extraction Advantages Disadvantages
How to optimize the extraction salt in QuEChERS?
– Original, Non-buffered: 4 g MgSO4, 1 g NaCl – AOAC: 6 g MgSO4,1.5 g NaAc – EN: 4 g MgSO4, 1 g NaCl, 1 g NaCitrate, 0.5 g disodium citrate sesquihydrate 15 Use one dSPE mixture and keep this part the same for the extraction salt optimization TIP! QuEChERS Extraction Optimization Summary
How many ml of solvent are used in QuEChERS?
6 x faster Solvent Used (mL) per sample 90 mL 10-15mL 9 x less solvent Chlorinated Waste (mL) 30 mL none safer, greener, less costly Glassware/ specialized equipment Clean Separatory funnels, water bath, 200mL containers, evaporator, etc.